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Santa Cruz Biotechnology mouse anti vsx2
Mouse Anti Vsx2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 184 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti vsx2/product/Santa Cruz Biotechnology
Average 94 stars, based on 184 article reviews

mouse anti vsx2 - by Bioz Stars, 2026-05

94/100 stars

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Journal: The Journal of General Physiology

Article Title: ERG responses to high-frequency flickers require FAT3 signaling in mouse retinal bipolar cells

doi: 10.1085/jgp.202413642

Figure Lengend Snippet: Key resources table

Article Snippet: Mouse anti-VSX2 (Chx10) , Santa Cruz Biotechnology , Cat#sc-365519; RRID:AB_10842442.

Techniques: RNAscope, Multiplex Assay, In Situ Hybridization, Recombinant, Plasmid Preparation

BC and cones numbers, visualization of Grik1 + BCs morphology via injection of an AVV-Grik1-GFP virus in Fat3 mutants (Related to and ). (A) ARR3 immunohistochemistry of WT retinas. (A′) shows ARR3 staining together with DAPI staining. (B) ARR3 immunohistochemistry of Fat3 ∆TM/∆TM retinas. (B′) shows ARR3 staining together with DAPI staining. (C) VSX2 immunostaining of WT retinas. (D) VSX2 immunostaining of Fat3 ∆TM/∆TM retinas. (E) Quantification of number of cones marked by ARR3, as shown in A and B. WT controls: 27.77 ± 0.907 ( n = 4 animals, 13 retinal regions); Fat3 ∆TM/∆TM : 29.00 ± 0.593 ( n = 4 animals, 14 retinal regions). Each data point corresponds to a retinal region, color-coded by animal, analyzed using a nested two-tailed test. (F) Quantification of thickness of area occupied by VSX2 staining, as shown in C and D. WT controls: 21.09 ± 1.045 ( n = 4 animals, 15 retinal regions); Fat3 ∆TM/∆TM : 21.59 ± 0.969 ( n = 4 animals, 15 retinal regions). Each data point corresponds to a retinal region, color-coded by animal, analyzed using a nested two-tailed test. (G) Examples of retinal sections showing cells expressing GFP under the control of the Grik1 enhancer. The GFP reporter was introduced through in vivo injection of AAV8-Grik1-GFP. WT retinas are shown in G. (H) Fat3 ∆TM/∆TM retinas injected with AAV8-Grik1-GFP. The OPL is indicated with a yellow arrowhead and the OMPL, labeled with VGAT staining, is marked with a yellow arrow. Scale bar: 20 µm. (I) Quantification of Grik1 + BC dendrites in the OPL (WT Controls: 100%; Fat3 ∆TM/∆TM : 100%), axons in the IPL only (WT Controls: 100%; Fat3 ∆TM/∆TM : 44 ± 18.6%), and axons in the OMPL or in the OMPL and IPL (WT Controls: 0%; Fat3 ∆TM/∆TM : 56 ± 18.6%). Controls: n = 5 animals, 12 cells, Fat3 ∆TM/∆TM : n = 7 animals, 22 cells. Mann–Whitney test. Error bars: SEM.

Journal: The Journal of General Physiology

Article Title: ERG responses to high-frequency flickers require FAT3 signaling in mouse retinal bipolar cells

doi: 10.1085/jgp.202413642

Figure Lengend Snippet: BC and cones numbers, visualization of Grik1 + BCs morphology via injection of an AVV-Grik1-GFP virus in Fat3 mutants (Related to and ). (A) ARR3 immunohistochemistry of WT retinas. (A′) shows ARR3 staining together with DAPI staining. (B) ARR3 immunohistochemistry of Fat3 ∆TM/∆TM retinas. (B′) shows ARR3 staining together with DAPI staining. (C) VSX2 immunostaining of WT retinas. (D) VSX2 immunostaining of Fat3 ∆TM/∆TM retinas. (E) Quantification of number of cones marked by ARR3, as shown in A and B. WT controls: 27.77 ± 0.907 ( n = 4 animals, 13 retinal regions); Fat3 ∆TM/∆TM : 29.00 ± 0.593 ( n = 4 animals, 14 retinal regions). Each data point corresponds to a retinal region, color-coded by animal, analyzed using a nested two-tailed test. (F) Quantification of thickness of area occupied by VSX2 staining, as shown in C and D. WT controls: 21.09 ± 1.045 ( n = 4 animals, 15 retinal regions); Fat3 ∆TM/∆TM : 21.59 ± 0.969 ( n = 4 animals, 15 retinal regions). Each data point corresponds to a retinal region, color-coded by animal, analyzed using a nested two-tailed test. (G) Examples of retinal sections showing cells expressing GFP under the control of the Grik1 enhancer. The GFP reporter was introduced through in vivo injection of AAV8-Grik1-GFP. WT retinas are shown in G. (H) Fat3 ∆TM/∆TM retinas injected with AAV8-Grik1-GFP. The OPL is indicated with a yellow arrowhead and the OMPL, labeled with VGAT staining, is marked with a yellow arrow. Scale bar: 20 µm. (I) Quantification of Grik1 + BC dendrites in the OPL (WT Controls: 100%; Fat3 ∆TM/∆TM : 100%), axons in the IPL only (WT Controls: 100%; Fat3 ∆TM/∆TM : 44 ± 18.6%), and axons in the OMPL or in the OMPL and IPL (WT Controls: 0%; Fat3 ∆TM/∆TM : 56 ± 18.6%). Controls: n = 5 animals, 12 cells, Fat3 ∆TM/∆TM : n = 7 animals, 22 cells. Mann–Whitney test. Error bars: SEM.

Article Snippet: Mouse anti-VSX2 (Chx10) , Santa Cruz Biotechnology , Cat#sc-365519; RRID:AB_10842442.

Techniques: Injection, Virus, Immunohistochemistry, Staining, Immunostaining, Two Tailed Test, Expressing, Control, In Vivo, Labeling, MANN-WHITNEY

In situ hybridization of Grik1 and Grm6 and immunostaining of GRM6 upon loss of Fat3 in mouse retina (Related to ). (A–A″) VSX2 immunostaining (A) after in situ hybridization for Grik1 (A′) and Grm6 (A″) in WT retina. (B–B″) VSX2 immunostaining (B) after in situ hybridization for Grik1 (B′) and Grm6 (B″) in Fat3 ∆TM/∆TM retina. (C) Quantification of Grik1 RNA mean fluorescence intensity in bipolar cells. WT: 1.00 ± 0.03, n = 4 animals, 16 retinal regions; Fat3 ∆TM/∆TM : 0.75 ± 0.04, n = 4 animals, 17 retinal regions. Each data point corresponds to a retinal region, color-coded by animal, analyzed using a nested two-tailed test. (D) Quantification of Grm6 RNA mean fluorescence intensity in bipolar cells. WT: 1.00 ± 0.02, n = 4 animals, from 16 retinal regions; Fat3 ∆TM/∆TM : 0.98 ± 0.02, n = 4 animals, from 17 retinal regions. Each data point corresponds to a retinal region, color-coded by animal, analyzed using a nested two-tailed test. (E) Immunostaining for GRM6 in WT retinas. (F) Immunostaining for GRM6 in Fat3 ∆TM/∆TM retinas. (E′ and F′) Cone arrestin (ARR3) labels the cone photoreceptors endings in the OPL in E′ and F′. (G) Immunostaining for GRM6 in WT retinas, littermates of Fat3 ∆ICD-GFP/∆ICD-GFP animals. (H) Immunostaining for GRM6 in Fat3 ∆ICD-GFP/∆ICD-GFP retinas. Cone arrestin (ARR3) labels the cone photoreceptors endings in the OPL in G′ and H′. (I) Quantification of GRM6 integrated intensity in the OPL. WT controls: 1.00 ± 0.08 ( n = 8 animals, 35 retinal regions); Fat3 ∆TM/∆TM : 1.15 ± 0.09 ( n = 9 animals, 41 retinal regions). Each data point corresponds to a retinal region, color-coded by animal, nested two-tailed test. (J) Quantification of GRM6 integrated intensity in the OPL. WT Controls: 1.00 ± 0.09 ( n = 5 animals, 21 retinal regions); Fat3 ∆ICD/∆ICD : 0.86 ± 0.07 ( n = 5 animals, 22 retinal regions). Each data point corresponds to a retinal region, color-coded by animal, analyzed using a nested two-tailed test. (K) Flicker ERG amplitude at 30 Hz for WT (18.70 ± 2.05 µV n = 6 eyes) and Ptprs −/− retinas (24.50 ± 2.86 µV, n = 6 eyes). (L) Flicker ERG implicit time at 20 Hz for WT (34.3 ± 0.6 ms, n = 6 eyes) and Ptprs −/− retinas (34.5 ± 1.4 ms, n = 6 eyes). (M) Representative flicker ERG raw traces of WT control and Ptprs −/− eyes elicited by 3.162 cd s/m 2 flashes at 20 and 30 Hz frequencies. (N) Quantification of step ERG d-wave amplitudes of WT (55.58 ± 6.43 µV n = 6 eyes) and Ptprs −/− (46.40 ± 5.94 µV n = 6 eyes) elicited by a 3-s step of light at 1,000 cd/m 2 intensity. (O) Representative step ERG raw traces of WT ( n = 6) control and Ptprs −/− ( n = 6) eyes elicited by a 3-s step light at 1,000 cd/m 2 intensity. Scale bars: 20 µm. Error bars: SEM.

Journal: The Journal of General Physiology

Article Title: ERG responses to high-frequency flickers require FAT3 signaling in mouse retinal bipolar cells

doi: 10.1085/jgp.202413642

Figure Lengend Snippet: In situ hybridization of Grik1 and Grm6 and immunostaining of GRM6 upon loss of Fat3 in mouse retina (Related to ). (A–A″) VSX2 immunostaining (A) after in situ hybridization for Grik1 (A′) and Grm6 (A″) in WT retina. (B–B″) VSX2 immunostaining (B) after in situ hybridization for Grik1 (B′) and Grm6 (B″) in Fat3 ∆TM/∆TM retina. (C) Quantification of Grik1 RNA mean fluorescence intensity in bipolar cells. WT: 1.00 ± 0.03, n = 4 animals, 16 retinal regions; Fat3 ∆TM/∆TM : 0.75 ± 0.04, n = 4 animals, 17 retinal regions. Each data point corresponds to a retinal region, color-coded by animal, analyzed using a nested two-tailed test. (D) Quantification of Grm6 RNA mean fluorescence intensity in bipolar cells. WT: 1.00 ± 0.02, n = 4 animals, from 16 retinal regions; Fat3 ∆TM/∆TM : 0.98 ± 0.02, n = 4 animals, from 17 retinal regions. Each data point corresponds to a retinal region, color-coded by animal, analyzed using a nested two-tailed test. (E) Immunostaining for GRM6 in WT retinas. (F) Immunostaining for GRM6 in Fat3 ∆TM/∆TM retinas. (E′ and F′) Cone arrestin (ARR3) labels the cone photoreceptors endings in the OPL in E′ and F′. (G) Immunostaining for GRM6 in WT retinas, littermates of Fat3 ∆ICD-GFP/∆ICD-GFP animals. (H) Immunostaining for GRM6 in Fat3 ∆ICD-GFP/∆ICD-GFP retinas. Cone arrestin (ARR3) labels the cone photoreceptors endings in the OPL in G′ and H′. (I) Quantification of GRM6 integrated intensity in the OPL. WT controls: 1.00 ± 0.08 ( n = 8 animals, 35 retinal regions); Fat3 ∆TM/∆TM : 1.15 ± 0.09 ( n = 9 animals, 41 retinal regions). Each data point corresponds to a retinal region, color-coded by animal, nested two-tailed test. (J) Quantification of GRM6 integrated intensity in the OPL. WT Controls: 1.00 ± 0.09 ( n = 5 animals, 21 retinal regions); Fat3 ∆ICD/∆ICD : 0.86 ± 0.07 ( n = 5 animals, 22 retinal regions). Each data point corresponds to a retinal region, color-coded by animal, analyzed using a nested two-tailed test. (K) Flicker ERG amplitude at 30 Hz for WT (18.70 ± 2.05 µV n = 6 eyes) and Ptprs −/− retinas (24.50 ± 2.86 µV, n = 6 eyes). (L) Flicker ERG implicit time at 20 Hz for WT (34.3 ± 0.6 ms, n = 6 eyes) and Ptprs −/− retinas (34.5 ± 1.4 ms, n = 6 eyes). (M) Representative flicker ERG raw traces of WT control and Ptprs −/− eyes elicited by 3.162 cd s/m 2 flashes at 20 and 30 Hz frequencies. (N) Quantification of step ERG d-wave amplitudes of WT (55.58 ± 6.43 µV n = 6 eyes) and Ptprs −/− (46.40 ± 5.94 µV n = 6 eyes) elicited by a 3-s step of light at 1,000 cd/m 2 intensity. (O) Representative step ERG raw traces of WT ( n = 6) control and Ptprs −/− ( n = 6) eyes elicited by a 3-s step light at 1,000 cd/m 2 intensity. Scale bars: 20 µm. Error bars: SEM.

Article Snippet: Mouse anti-VSX2 (Chx10) , Santa Cruz Biotechnology , Cat#sc-365519; RRID:AB_10842442.

Techniques: In Situ Hybridization, Immunostaining, Fluorescence, Two Tailed Test, Control

Fat3 RNA is enriched in OFF-cone bipolar cells. (A) Schematic representation of the retina highlighting the position of bipolar cell bodies, as stained by VSX2. (B) In situ hybridization for Fat3 RNA in WT P22 retinas. (C) In situ hybridization for Fat3 RNA in Fat3 ∆TM/∆TM P22 retinal tissue. (B′ and C′) In B and C the RNA puncta are shown in white and the yellow brackets indicate the area of VSX2 + cell bodies (cyan, B′ and C′). Yellow dashed lines demark the inner nuclear layer (INL) and the outer misplaced plexiform layer (OMPL) in Fat3 ∆TM/∆TM tissue. The squares demark the insets seen in B′ and C′ at higher magnification. VSX2 protein is seen in cyan. (D) Hybridization chain reaction-immunohistochemistry (HCR-IHC) of FAT3 in wild type retinas. (D′) Inset demarked in a yellow box in D is shown at higher magnification in D′. (E) HCR-IHC of FAT3 in Fat3 ∆TM/∆TM mutant retinas. (E′) Inset demarked in a yellow box in E is shown at higher magnification in E′. (F) Schematic representation of Grik1 and Grm6 RNA enrichment in bipolar cells, according to data in . (G and G′) in situ hybridization of Grik1 RNA (magenta) and Grm6 RNA (yellow, G′) with immunostaining for VSX2 (cyan). (G″ and G‴) The insets in G and G′ are shown at a higher magnification in G″ and G‴. (H) Triple in situ hybridization of Fat3 (white), Grik1 (magenta), and Grm6 (yellow) RNA. (I–I‴) Inset in H is seen at higher magnification in I–I‴. (I) Higher magnification of inset shown in H. Yellow dashed lines in I″ and I‴ demark Grik1 RNA + cell bodies. Fat3 RNA (white) is shown together with Grm6 (yellow) RNA in I′, with Grik1 (magenta) RNA in I″ and alone in I‴. Scale bars: 20 µm.

Journal: The Journal of General Physiology

Article Title: ERG responses to high-frequency flickers require FAT3 signaling in mouse retinal bipolar cells

doi: 10.1085/jgp.202413642

Figure Lengend Snippet: Fat3 RNA is enriched in OFF-cone bipolar cells. (A) Schematic representation of the retina highlighting the position of bipolar cell bodies, as stained by VSX2. (B) In situ hybridization for Fat3 RNA in WT P22 retinas. (C) In situ hybridization for Fat3 RNA in Fat3 ∆TM/∆TM P22 retinal tissue. (B′ and C′) In B and C the RNA puncta are shown in white and the yellow brackets indicate the area of VSX2 + cell bodies (cyan, B′ and C′). Yellow dashed lines demark the inner nuclear layer (INL) and the outer misplaced plexiform layer (OMPL) in Fat3 ∆TM/∆TM tissue. The squares demark the insets seen in B′ and C′ at higher magnification. VSX2 protein is seen in cyan. (D) Hybridization chain reaction-immunohistochemistry (HCR-IHC) of FAT3 in wild type retinas. (D′) Inset demarked in a yellow box in D is shown at higher magnification in D′. (E) HCR-IHC of FAT3 in Fat3 ∆TM/∆TM mutant retinas. (E′) Inset demarked in a yellow box in E is shown at higher magnification in E′. (F) Schematic representation of Grik1 and Grm6 RNA enrichment in bipolar cells, according to data in . (G and G′) in situ hybridization of Grik1 RNA (magenta) and Grm6 RNA (yellow, G′) with immunostaining for VSX2 (cyan). (G″ and G‴) The insets in G and G′ are shown at a higher magnification in G″ and G‴. (H) Triple in situ hybridization of Fat3 (white), Grik1 (magenta), and Grm6 (yellow) RNA. (I–I‴) Inset in H is seen at higher magnification in I–I‴. (I) Higher magnification of inset shown in H. Yellow dashed lines in I″ and I‴ demark Grik1 RNA + cell bodies. Fat3 RNA (white) is shown together with Grm6 (yellow) RNA in I′, with Grik1 (magenta) RNA in I″ and alone in I‴. Scale bars: 20 µm.

Article Snippet: Mouse anti-VSX2 (Chx10) , Santa Cruz Biotechnology , Cat#sc-365519; RRID:AB_10842442.

Techniques: Staining, In Situ Hybridization, Hybridization, Immunohistochemistry, Mutagenesis, Immunostaining

Journal: Cell reports

Article Title: Global Analysis of Intercellular Homeodomain Protein Transfer

doi: 10.1016/j.celrep.2019.06.056

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse monoclonal anti-VSX2 (E-12) antibody , Santa Cruz Biotechnology , RRID: AB_10842442.

Techniques: Recombinant, In Vitro, Transfection, Protease Inhibitor, Control, Plasmid Preparation, Software, Expressing, Fluorescence

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